Caspase 3 ELISA Kit

Catalog No. ABIN5524447

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Caspase 3. There is 1 publication available.

Quick Overview for Caspase 3 ELISA Kit (ABIN5524447)

Target: Caspase 3 (CASP3)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details Caspase 3 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: For quantitative detection of Casp3 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Casp3. No significant cross-reactivity or interference between Casp3 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between Casp3 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.094 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for Caspase 3 ELISA Kit (ABIN5524447)

De Cillà, Vezzola, Farruggio, Vujosevic, Clemente, Raina, Mary, Casini, Rossetti, Avagliano, Martinelli, Bulfamante, Grossini: "The subthreshold micropulse laser treatment of the retina restores the oxidant/antioxidant balance and counteracts programmed forms of cell death in the mice eyes." in: Acta ophthalmologica, (2018) (PubMed).

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Casp3

Background: Casp3/CASP3/CPP32/CPP32B/SCA-1/Apoptain/Yama/Apopain/LICE-1/YAMA/Apopain/apoptosis-related cysteine protease/CASP-3/caspase 3, apoptosis-related cysteine peptidase/caspase-3/CPP-32/CPP32/SREBP cleavage activity 1/Cysteine protease CPP32/PARP cleavage prot

UniProt: P70677

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation