Osteopontin ELISA Kit

Catalog No. ABIN5525065

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Osteopontin.

Quick Overview for Osteopontin ELISA Kit (ABIN5525065)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details Osteopontin ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: For quantitative detection of OPN in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of OPN. No significant cross-reactivity or interference between OPN and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between OPN and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.094 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: OPN

Background: OPN(Osteopontin)/Eta-1/Spp1/BNSP/Bone sialoprotein 1/MGC110940/Nephropontin/secreted phosphoprotein-1(osteopontin, bone sialoprotein)/SPP-1/SPP1/CALPHA1 fusion/Urinary stone protein/uropontin

UniProt: P10451

Pathways: Regulation of Cell Size