PCK1 ELISA Kit

Catalog No. ABIN5525220

Human PCK1 ELISA Kit Colorimetric assay for quantification of Human PCK1.

Quick Overview for PCK1 ELISA Kit (ABIN5525220)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details PCK1 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: For quantitative detection of PCK1 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of PCK1. No significant cross-reactivity or interference between PCK1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between PCK1 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.094 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1

Background: PCK1(Phosphoenolpyruvate Carboxykinase 1)/PEPCK1/PEPCK-C/PEPCKC/PEPCK-CPEP carboxykinase/phosphoenolpyruvate carboxykinase 1(soluble)/phosphoenolpyruvate carboxykinase, cytosolic/phosphoenolpyruvate carboxykinase, cytosolic [GTP]/Phosphoenolpyruvate carbo

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis