Pro-Cathepsin B ELISA Kit

Catalog No. ABIN5526685

This Human Pro-Cathepsin B ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Pro-Cathepsin B.

Quick Overview for Pro-Cathepsin B ELISA Kit (ABIN5526685)

Target: Pro-Cathepsin B

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 61 pg/mL-15 ng/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details Pro-Cathepsin B ELISA Kit

Minimum Detection Limit: 61 ng/mL

Purpose: Human Pro-Cathepsin B ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Pro-Cathepsin B.

Sensitivity: 61 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Reagent Preparation:

Recommended Dilution for serum and plasma samples4 fold

Restrictions: For Research Use only

Handling

Storage: -20 °C

Expiry Date: 6 months

Target Details for Pro-Cathepsin B

Target: Pro-Cathepsin B

Gene ID: 1508

UniProt: P07858