AZGP1 ELISA Kit

Catalog No. ABIN5564198

Human AZGP1 ELISA Kit Colorimetric assay for quantification of Human AZGP1.

Quick Overview for AZGP1 ELISA Kit (ABIN5564198)

Target: AZGP1 (alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.9375-60 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details AZGP1 ELISA Kit

Minimum Detection Limit: 0.9375 ng/mL

Analytical Method: Quantitative

Specificity: Detects human Zinc-alpha-2-glycoprotein.

Cross-Reactivity: Human

No Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): Does not detect mouse Zinc-alpha-2-glycoprotein.

Sensitivity: 0.23 ng/mL

Characteristics: Zinc-alpha-2-glycoprotein (human) TurboELISA Kit
This assay is a less than 1 hour sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human Zinc-alpha-2-glycoprotein in biological fluids. Zinc-alpha-2-glycoprotein (ZAG) was first identified in the 1960s, and it's name derives from its precipitation from human plasma upon the addition of zinc salts. ZAG has since been found in secretory epithelial cells and in a range of body fluids. ZAG is identical to a lipid mobilizing factor isolated from the urine of patients with cancer cachexia and stimulates lipolysis in in vitro and in vivo experiments. Due to its expression in, and secretion from adipocytes, ZAG is considered an adipokine. Recently, the clinical significance of ZAG has been clarified. ZAG expression in adipocytes is inversely related to fat mass, thus it is intimately involved in the maintenance of body weight in mice and humans. Epidemiological studies have uncovered an association between ZAG and plasma cholesterol. The non-synonymous single nucleotide polymorphism rs4215 in ZAG is associated with plasma cholesterol and obesity. Structurally ZAG possesses a class I major histocompatibility complex (MHC) protein fold. It is distinct from other members of this protein family in that it is soluble, rather than being anchored to plasma membranes, and it associates with prolactin inducible protein rather than beta2-microglobulin. Similar to peptide antigen-presenting class I MHC molecules, ZAG possesses an open apical groove between its alpha1 and alpha2 domain helices.

Application Details

Comment:

TRADEMARKS - TurboELISA™ and Turbo-coated Plate™ are Trademarks of Adipogen Life Sciences.

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: +4°C
12 months after the day of manufacturing. See expiry date on ELISA Kit box.

Target Details for AZGP1

Target: AZGP1 (alpha-2-Glycoprotein 1, Zinc-Binding (AZGP1))

Alternative Name: Zinc-alpha-2-glycoprotein

UniProt: P25311

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process