FABP5 ELISA Kit
Quick Overview for FABP5 ELISA Kit (ABIN5564619)
Target
See all FABP5 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Brand
- AssayMax™
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Analytical Method
- Quantitative
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Material not included
- Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)
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Fatty Acid Binding Protein 5 (Psoriasis-Associated) (FABP5) ELISA Kit
FABP5 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fatty Acid Binding Protein 5 (Psoriasis-Associated) (FABP5) ELISA KitFABP5 Reactivity: Mouse Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Fatty Acid Binding Protein 5 (Psoriasis-Associated) (FABP5) ELISA KitFABP5 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Milk, Tissue Homogenate
Fatty Acid Binding Protein 5 (Psoriasis-Associated) (FABP5) ELISA KitFABP5 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Milk, Plasma, Serum, Tissue Homogenate
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Assay Time
- 5 h
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Plate
- Pre-coated
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Calculation of Results
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- Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
- To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
- Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.
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Restrictions
- For Research Use only
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Handling Advice
- This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2
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Storage
- 4 °C,-20 °C
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- FABP5 (Fatty Acid Binding Protein 5 (Psoriasis-Associated) (FABP5))
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Alternative Name
- Fatty Acid-Binding Protein 5 (FABP5)
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Gene ID
- 2171
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UniProt
- Q01469
Target See all FABP5 ELISA Kits
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