Biotin ELISA Kit

Catalog No. ABIN5564656

This Various Species Biotin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Various Species Biotin.

Quick Overview for Biotin ELISA Kit (ABIN5564656)

Target: Biotin

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 32 pg/mL - 20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details Biotin ELISA Kit

Minimum Detection Limit: 32 pg/mL

Analytical Method: Quantitative

Specificity:

This assay has high sensitivity and excellent specificity for detection of Vitamin B7 (VB7).
No significant cross-reactivity or interference between Vitamin B7 (VB7) and analogues was observed.

Sensitivity: 13 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Application Details

Sample Volume: 50 μL

Assay Time: 2 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards,
2. Add 50μL standard or sample to each well.
   Then add 50μL prepared Detection Reagent A immediately.
   Shake and mix. Incubate 1 hour at 37 °C,
3. Aspirate and wash 5 times,
4. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
5. Add 50μL Stop Solution. Read at 450 nm immediately.

Assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin B7 (VB7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin B7 (VB7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: 4 °C/-20 °C

Storage Comment:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Expiry Date: 6 months

Target Details for Biotin

Target: Biotin

Alternative Name: Vitamin B7 (VB7)