HABA Biotin Quantitation Kit
EAA
Colorimetric
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- Target See all Biotin products
- Biotin
- Detection Method
- Colorimetric
- Conjugate
- Biotin
- Application
- Enzyme Activity Assay (EAA)
- Characteristics
- The HABA Biotin Quantitation Kit provides a convenient method for estimating the molar ratio of biotin to protein in biotin-protein conjugates or for quantitating biotin concentration in a solution. The assay employs HABA (4'-hydroxyazobenzene-2-carboxylic acid), a reagent that shows dramatic spectral changes when bound to avidin. Biotin easily displaces HABA from the HABA/Avidin complex, resulting in a decrease of absorption at 500 nm. The kit is best used to determine biotin concentration in the range from 2 to 16 µM. The assay can be performed in a cuvette or microplate format.
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Biotin ELISA Kit
Reactivity: Various Species Colorimetric Competition ELISA 32 pg/mL - 20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Comment
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Enzymatic Activity Assay kit
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store Component A at -20 °C. Store all other components at 4 °C
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- Target
- Biotin
- Abstract
- Biotin Products
- Target Type
- Chemical
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