Exosome Purification and Analysis Kit (Cell culture)
Quick Overview for Exosome Purification and Analysis Kit (Cell culture) (ABIN5633279)
Reactivity
Detection Method
Application
Sample Type
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Conjugate
- PE
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Purpose
- Exosome purification and FACS analysis kit of exosomes derived from cell culture
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Brand
- ExoStep™
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Characteristics
- The kit is a simple immunobead assay for isolation/detection of exosome, using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody. The kit provides reproducible results and can be run in parallel to exosome immunophenotyping.
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Components
- CD63+ capture beads Anti-CD9 biotin Streptavidin-Phycoerythrin (PE) Assay Buffer 10X
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Material not included
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• Pre-enriched exosomes by ultra-centrifugation. • Magnetic Rack, MagneSphere(R) Mag. Sep. Stand 12-hole, 12x75mm (PROMEGA, Ref Z5343). • 12x75 mm Polystyrene Round Bottom Tubes (cytometer
- tubes
). • Sterile syringe filter with a 0.22 μm pore. (EMD Millipore, Ref SLGSV255F) • Syringe of adequate volume
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Amyloid beta 1-40 (Abeta 1-40) ELISA KitAbeta 1-40 Reactivity: Human Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 Reactivity: Human Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Lipopolysaccharides (LPS) ELISA KitReactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivity: Human AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 Reactivity: Human Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
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Application Notes
- Isolate and purify exosomes by FACS with reproducible results. You can calculate the concentration of protein easily and without other protein contaminations. It allows also immunophenotyping of the captured exosomes
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Comment
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Very reproducible and faster than WB or TME.3 hours for enrichement the sample+ Overnight Incubation+ 1 hour of protocol
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Sample Volume
- 50 μL
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Assay Time
- 4 h
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Reagent Preparation
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Reagents ready to use.
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Restrictions
- For Research Use only
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Buffer
- The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Store in the dark at 2-8 °C
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Background
- Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB)1, with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases.
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