Glutamic Acid Decarboxylase ELISA Kit

Catalog No. ABIN5654675

Human Glutamic Acid Decarboxylase ELISA Kit Colorimetric assay for quantification of Human Glutamic Acid Decarboxylase.

Quick Overview for Glutamic Acid Decarboxylase ELISA Kit (ABIN5654675)

Target: Glutamic Acid Decarboxylase (GAD)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Glutamic Acid Decarboxylase ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamic Acid Decarboxylase (GAD). No significant cross-reactivity or interference between Glutamic Acid Decarboxylase (GAD) and analogues was observed.

Sensitivity: 0.59 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Glutamic Acid Decarboxylase (GAD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Glutamic Acid Decarboxylase (GAD) and unlabeled Glutamic Acid Decarboxylase (GAD) (Standards or samples) with the pre-coated antibody specific to Glutamic Acid Decarboxylase (GAD). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Glutamic Acid Decarboxylase (GAD) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glutamic Acid Decarboxylase (GAD) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamic Acid Decarboxylase (GAD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamic Acid Decarboxylase (GAD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Glutamic Acid Decarboxylase

Target: Glutamic Acid Decarboxylase (GAD)

Alternative Name: Glutamic Acid Decarboxylase

Background:

Gene Name: Glutamic Acid Decarboxylase

Gene Aliases: Glutamate Decarboxylase