IgG1 ELISA Kit

Catalog No. ABIN5655306

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat IgG1.

Quick Overview for IgG1 ELISA Kit (ABIN5655306)

Target: IgG1

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 49.38 ng/mL - 4000 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details IgG1 ELISA Kit

Minimum Detection Limit: 49.38 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G1 (IgG1). No significant cross-reactivity or interference between Immunoglobulin G1 (IgG1) and analogues was observed.

Sensitivity: 18.56 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Immunoglobulin G1 (IgG1) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G1 (IgG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G1 (IgG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G1 (IgG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for IgG1

Target: IgG1

Alternative Name: Immunoglobulin G1

Target Type: Antibody

Background:

Gene Name: Immunoglobulin G1

Gene Aliases: IGHG1, Ig Gamma-1 Chain C Region, Immunoglobulin Heavy Constant Gamma 1, G1m Marker, Immunoglobulin Gm1