NKAP ELISA Kit

Catalog No. ABIN5674704

Mouse NKAP ELISA Kit Colorimetric assay for quantification of Mouse NKAP.

Quick Overview for NKAP ELISA Kit (ABIN5674704)

Target: NKAP (NFKB Activating Protein (NKAP))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Product Details NKAP ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Analytical Method: Quantitative

Sensitivity: 11.3 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for NKAP

Target: NKAP (NFKB Activating Protein (NKAP))

Background: Alternative name: NF Kappa B Activating Protein

Gene ID: 67050

UniProt: Q9D0F4

Pathways: Stem Cell Maintenance, The Global Phosphorylation Landscape of SARS-CoV-2 Infection