SREBF1 ELISA Kit
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- SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.39 ng/mL - 25 ng/mL
- Minimum Detection Limit
- 0.39 ng/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 0144 ng/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA Kit
SREBF1 Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA KitSREBF1 Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA KitSREBF1 Reactivity: Rat Colorimetric Sandwich ELISA 62.5-4000 pg/mL Plasma, Serum, Tissue Homogenate
Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA KitSREBF1 Reactivity: Human Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all SREBF1 ELISA Kits
- SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))
- Abstract
- SREBF1 Products
- Background
- Alternative name: SREBP1, Sterol Regulatory Element-Binding Protein 1, Adipocyte determination- and differentiation-dependent factor 1, ADD1
- Gene ID
- 78968
- UniProt
- P56720
- Pathways
- AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha
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