CAT2 ELISA Kit
Human CAT2 ELISA Kit Colorimetric assay for quantification of Human CAT2.
Catalog No. ABIN5674785
Quick Overview for CAT2 ELISA Kit (ABIN5674785)
Target
See all CAT2 products
CAT2
(Cationic Amino Acid Transporter 2 (CAT2))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.156 ng/mL - 10 ng/mL
Application
ELISA
Sample Type
Tissue Homogenate
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Minimum Detection Limit
- 0.156 ng/mL
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Analytical Method
- Quantitative
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Sensitivity
- 0054 ng/mL
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Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual -
Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
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Cationic Amino Acid Transporter 2 (CAT2) ELISA Kit
CAT2 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Assay Time
- 1 - 4.5 h
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Plate
- Pre-coated
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Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Expiry Date
- 12 months
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- CAT2 (Cationic Amino Acid Transporter 2 (CAT2))
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Background
- Alternative name: SLC7A2, ATRC2, CAT-2, HCAT2, Low Affinity Cationic Amino Acid Transporter 2, Solute Carrier Family 7 Member 2,Cationic Amino Acid Transporter,y+ System
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Gene ID
- 6542
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UniProt
- P52569
Target See all CAT2 products
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