PDE3B ELISA Kit

Catalog No. ABIN5674920

Human PDE3B ELISA Kit Colorimetric assay for quantification of Human PDE3B.

Quick Overview for PDE3B ELISA Kit (ABIN5674920)

Target: PDE3B (phosphodiesterase 3B, CGMP-Inhibited (PDE3B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details PDE3B ELISA Kit

Minimum Detection Limit: 78.125 pg/mL

Analytical Method: Quantitative

Sensitivity: 26.35 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for PDE3B

Target: PDE3B (phosphodiesterase 3B, CGMP-Inhibited (PDE3B))

Alternative Name: Phosphodiesterase 3B, cGMP Inhibited (PDE3B)

Background: Alternative name: HcGIP1, cGIPDE1, CGIPDE1, CGIP1, CGI-PDE B, Cyclic GMP-inhibited phosphodiesterase B

Gene ID: 5140

UniProt: Q13370

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, cAMP Metabolic Process