PKC theta FLISA Kit

Catalog No. ABIN5691913

This Fluorometric ELISA kit is designed for the quantitative measurement of Human, Mouse and Rat PKC theta in Cell Culture Cells.

Quick Overview for PKC theta FLISA Kit (ABIN5691913)

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Binding Specificity: pThr538

Reactivity: Human, Mouse, Rat

Detection Method: Fluorometric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details PKC theta FLISA Kit

Purpose: The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.

Brand: CytoFluor™

Analytical Method: Qualitative

Characteristics: Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma

Components: 2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual

Application Details

Application Notes: Detection Range: At least 5000 cells/well

Assay Time: 4.5 h

Plate: Uncoated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C/6 Months

Expiry Date: 6 months

Target Details

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Alternative Name: PKC theta

Gene ID: 5588

UniProt: Q04759

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling