PI3K p85 alpha/gamma FLISA Kit

Catalog No. ABIN5691987

Human, Mouse and Rat PI3K p85 alpha/gamma ELISA Kit Fluorometric assay for quantification of Human, Mouse and Rat PI3K p85 alpha/gamma.

Quick Overview for PI3K p85 alpha/gamma FLISA Kit (ABIN5691987)

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Binding Specificity: pTyr199, pTyr467

Reactivity: Human, Mouse, Rat

Detection Method: Fluorometric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details PI3K p85 alpha/gamma FLISA Kit

Purpose: The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.

Analytical Method: Qualitative

Characteristics: Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma

Components: 2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual

Application Details

Application Notes: Detection Range: At least 5000 cells/well

Assay Time: 4.5 h

Plate: Uncoated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C/6 Months

Expiry Date: 6 months

Target Details

Target: PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))

Alternative Name: PI3-kinase p85-alpha/gamma

Gene ID: 5295

UniProt: P27986, Q92569