ARTN ELISA Kit

Catalog No. ABIN5692196

This Human ARTN ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human ARTN.

Quick Overview for ARTN ELISA Kit (ABIN5692196)

Target: ARTN (Artemin (ARTN))

Binding Specificity: AA 108-220

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details ARTN ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Artemin. 96wells/kit, with removable strips.

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli, Immunogen sequence: A108-G220

Sensitivity: < 2 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for ARTN

Target: ARTN (Artemin (ARTN))

Alternative Name: ARTN

Background:

Synonyms: Artemin, Enovin, Neublastin, ARTN, EVN

Tissue Specificity: Ubiquitous. Expressed at high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung and testis. Expressed at low levels in the brain.

Background: Artemin, also known as enovin or neublastin, is a protein that in humans is encoded by the ARTN gene. This gene encodes a secreted ligand of the glial cell line-derived neurotrophic factor (GDNF) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein signals through the RET receptor and GFR alpha 3 coreceptor, and supports the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. This protein has also been shown to promote tumor growth, metastasis, and drug resistance in mammary carcinoma.

Cellular Localisation: Secreted.

UniProt: Q5T4W7