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TFF3 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat TFF3.
Catalog No. ABIN5692207

Quick Overview for TFF3 ELISA Kit (ABIN5692207)

Target

See all TFF3 ELISA Kits
TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))

Binding Specificity

AA 23-81

Reactivity

  • 8
  • 5
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.2 pg/mL - 2000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Minimum Detection Limit

    31.2 pg/mL

    Purpose

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of rat TFF3. 96wells/kit, with removable strips.

    Brand

    PicoKine™

    Analytical Method

    Quantitative

    Specificity

    Expression system for standard: E.coli, Immunogen sequence: A23-F81

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate precoated with antibody
    lyophilized recombinant standard
    biotinylated antibody (dilution 1:100)
    Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
    Sample diluent buffer
    Antibody diluent buffer
    ABC diluent buffer
    TMB color developing agent
    TMB stop solution
    Adhesive cover
  • Assay Time

    0.5 h

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

    Expiry Date

    12 months
  • Target See all TFF3 ELISA Kits

    TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))

    Alternative Name

    Tff3

    Background

    Synonyms: Trefoil factor 3, Intestinal trefoil factor, rITF, Polypeptide P1.B, rP1.B, Tff3, Itf

    Tissue Specificity: Expressed in goblet cells of the intestines, and colon, in paraventricular hypothalamus and supraoptic nuclei. Weakly expressed in gastric epithelial cells (at protein level). Expressed by goblet cells of small and large intestinal epithelia, kidney and stomach. Expressed in the paraventricular hypothalamus, arcuate nucleus and amygdala of the brain. Weakly expressed in gastric epithelial cells.

    Background: Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

    Cellular Localisation: Secreted, extracellular space, extracellular matrix.

    UniProt

    Q03191
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