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TFF3 ELISA Kit

TFF3 Reactivity: Human AA 22-80 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Catalog No. ABIN1889396
  • Target See all TFF3 ELISA Kits
    TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))
    Binding Specificity
    AA 22-80
    Reactivity
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TFF3
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: E22-Y80
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: E22-Y80
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 P-type (trefoil) domain.

    Tissue Specificity: Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular and supraoptic nuclei (at protein level). .

    Plate
    Pre-coated
    Protocol
    human TFF3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFF3 has been precoated onto 96-well plates. Standards(E.coli, E22-Y80) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFF3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFF3 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TFF3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TFF3 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 164, Standard deviation: 7.38, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 425, Standard deviation: 22.1, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 631, Standard deviation: 25.87, CV(%): 4.1,
    • Sample 1: n=24, Mean(pg/ml): 152, Standard deviation: 9.6, CV(%): 6.3
    • Sample 2: n=24, Mean(pg/ml): 395, Standard deviation: 28, CV(%): 7.1
    • Sample 3: n=24, Mean(pg/ml): 640, Standard deviation: 37.76, CV(%): 5.9
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all TFF3 ELISA Kits
    TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))
    Alternative Name
    TFF3 (TFF3 Products)
    Synonyms
    TFF3 ELISA Kit, ITF ELISA Kit, P1B ELISA Kit, TFI ELISA Kit, mITF ELISA Kit, trefoil factor 3 ELISA Kit, trefoil factor 3, intestinal ELISA Kit, TFF3 ELISA Kit, Tff3 ELISA Kit
    Background

    Protein Function: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). .

    Background: Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

    Synonyms: Trefoil factor 3,Intestinal trefoil factor,hITF,Polypeptide P1.B,hP1.B,TFF3,ITF, TFI,

    Full Gene Name: Trefoil factor 3

    Cellular Localisation: Secreted, extracellular space, extracellular matrix . Cytoplasm.
    Gene ID
    7033
    UniProt
    Q07654
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