FABP3 ELISA Kit

Catalog No. ABIN5692217

The Rat FABP3 ELISA Kit (ABIN5692217) is a Colorimetric ELISA Kit designed to quantify Rat FABP3.

Quick Overview for FABP3 ELISA Kit (ABIN5692217)

Target: FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart (FABP3))

Binding Specificity: AA 2-133

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.7 pg/mL - 6000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details FABP3 ELISA Kit

Minimum Detection Limit: 93.7 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of rat FABP3. 96wells/kit, with removable strips.

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli, Immunogen sequence: A2-A133

Sensitivity: < 10 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 25 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for FABP3

Target: FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart (FABP3))

Alternative Name: Fabp3

Background:

Synonyms: Fatty acid-binding protein, heart, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, H-FABP, Fabp3

Tissue Specificity: Heart, but also skeletal muscle, kidney, brain and mammary gland.

Background: Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.

Cellular Localisation: Cytoplasm.

UniProt: P07483

Pathways: Monocarboxylic Acid Catabolic Process