TIM3 ELISA Kit

Catalog No. ABIN5692221

Mouse TIM3 ELISA Kit Colorimetric assay for quantification of Mouse TIM3.

Quick Overview for TIM3 ELISA Kit (ABIN5692221)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Binding Specificity: AA 20-193

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details TIM3 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse TIM-3/HAVCR2. 96wells/kit, with removable strips.

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO, Immunogen sequence: R20-A193

Sensitivity: < 10 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: Havcr2

Target Type: Virus

Background:

Synonyms: Hepatitis A virus cellular receptor 2 homolog, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, TIMD-3, T-cell immunoglobulin mucin receptor 3, TIM-3, T-cell membrane protein 3, Havcr2, Tim3, Timd3

Tissue Specificity: Expressed in T-helper type 1 lymphocytes. Not expressed by naive T-cells but up-regulated as they differentiate into T-helper-1 cells. Also expressed by differentiated type 1 CD8+ cytotoxic T-cells. Expressed on peritoneal exudate macrophages, monocytes, and splenic dendritic cells (DCs). Expression on natural killer (NK) cells is inversely associated with IFN-gamma production during the initial 24 h of LPS-induced endotoxic shock. Expressed on mast cells.

Background: Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in mouses is encoded by the HAVCR2 gene. The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.

Cellular Localisation: Isoform 1: Membrane, Single- pass type I membrane protein . Cell junction

UniProt: Q8VIM0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints