CRLF2 ELISA Kit

Catalog No. ABIN5692222

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse CRLF2.

Quick Overview for CRLF2 ELISA Kit (ABIN5692222)

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Binding Specificity: AA 20-233

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CRLF2 ELISA Kit

Minimum Detection Limit: 125 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CRLF2. 96wells/kit, with removable strips.

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO, Immunogen sequence: A20-L233

Sensitivity: < 10 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for CRLF2

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Alternative Name: Crlf2

Background:

Synonyms: Cytokine receptor-like factor 2, Cytokine receptor-like molecule 2, CRLM-2, Thymic stromal lymphopoietin protein receptor, TSLP receptor, Type I cytokine receptor delta 1, Crlf2, Crlm2, Tpte2, Tslpr

Tissue Specificity: High level of expression in liver, lung and testis. Also expressed in heart, brain, spleen, thymus and bone marrow. Highly expressed in progenitors and myeloid cells. Isoform 2 is expressed in primary hemotopoietic cells.

Background: Cytokine receptor-like factor 2, also known as TSLPR or CRL2, is a protein that in humans is encoded by the CRLF2 gene. It is mapped to Xp22.33. The protein encoded by this gene is a receptor for thymic stromal lymphopoietin(TSLP). Together with the interleukin 7 receptor(IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Two transcript variants encoding different isoforms have been found for this gene. In addition to it, it has been found that rearrangement of CRLF2 and JAK mutation together contribute to leukemogenesis in B-progenitor ALL.

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.

UniProt: Q8CII9