NPPA ELISA Kit

Catalog No. ABIN5692227

Human NPPA ELISA Kit Colorimetric assay for quantification of Human NPPA and has been mentioned in 1 publication.

Quick Overview for NPPA ELISA Kit (ABIN5692227)

Target: NPPA (Natriuretic Peptide A (NPPA))

Binding Specificity: AA 26-123

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details NPPA ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NPPA. 96wells/kit, with removable strips.

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli, Immunogen sequence: N26-R123

Sensitivity: < 10 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

References for NPPA ELISA Kit (ABIN5692227)

Yuan, Pan, Fu, Bi: "Effect of growth factors (BMP-4/7 & bFGF) on proliferation & osteogenic differentiation of bone marrow stromal cells." in: The Indian journal of medical research, Vol. 138, pp. 104-10, (2014) (PubMed).

Target Details for NPPA

Target: NPPA (Natriuretic Peptide A (NPPA))

Alternative Name: NPPA

Background:

Synonyms: Natriuretic peptides A, CDD-ANF, Cardiodilatin, CDD, Cardiodilatin-related peptide, CDP, Prepronatriodilatin, Atrial natriuretic factor, ANF, Atrial natriuretic peptide, ANP, NPPA, ANP, PND

Background: Atrial natriuretic peptide (ANP), also known as NPPA or PND, is a powerful vasodilator, and a protein (polypeptide) hormone secreted by heart muscle cells. This gene is mapped to 1p36.22. It is involved in the homeostatic control of body water, sodium, potassium and fat (adipose tissue). ANP is released by muscle cells in the upper chambers (atria) of the heart (atrial myocytes) in response to high blood volume. It acts to reduce the water, sodium and adipose loads on the circulatory system, thereby reducing blood pressure. ANP has exactly the opposite function of the aldosterone secreted by the zona glomerulosa in regard to its effect on sodium in the kidney - that is, aldosterone stimulates sodium retention and it generates sodium loss.

Cellular Localisation: Secreted.

UniProt: P01160