DKK4 ELISA Kit

Catalog No. ABIN5692231

This Colorimetric ELISA kit is designed for the quantitative measurement of Human DKK4.

Quick Overview for DKK4 ELISA Kit (ABIN5692231)

Target: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Binding Specificity: AA 19-224

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details DKK4 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DKK-4. 96wells/kit, with removable strips.

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO, Immunogen sequence: L19-L224

Sensitivity: < 10 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for DKK4

Target: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Alternative Name: DKK4

Background:

Synonyms: Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, Dickkopf-related protein 4 short form, DKK4

Tissue Specificity: Expressed in cerebellum, T-cells, esophagus and lung.

Background: DKK4 is a membersof the Dickkopf family which are negative regulators of the growth-promoting Wnt / beta-catenin signaling pathway. By FISH, the DKK4 gene was mapped to chromosome 8p11.2-p11.1. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. The expression of DKK4 was significantly reduced in hepatocellular carcinomas (HCCs), particularly in HCCs that showed cytoplasmic beta- catenin accumulation. DKK4 expression was also reduced in HCC cell lines. Ectopic expression of DKK4 in 2 HCC cell lines reduced beta-catenin and cyclin D1 protein levels and attenuated beta-catenin activity in reporter gene assays.

Cellular Localisation: Secreted.

UniProt: Q9UBT3

Pathways: WNT Signaling