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AMBP ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human AMBP.
Catalog No. ABIN5692240
$499.00
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 4 to 6 Business Days

Quick Overview for AMBP ELISA Kit (ABIN5692240)

Target

See all AMBP ELISA Kits
AMBP (alpha 1 Microglobulin/bikunin precursor (AMBP))

Binding Specificity

AA 206-352

Reactivity

  • 11
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  • 8
  • 5
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6 pg/mL - 1000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Minimum Detection Limit

    15.6 pg/mL

    Purpose

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Bikunin. 96wells/kit, with removable strips.

    Analytical Method

    Quantitative

    Specificity

    Expression system for standard: NSO, Immunogen sequence: A206-N352

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate precoated with antibody
    lyophilized recombinant standard
    biotinylated antibody (dilution 1:100)
    Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
    Sample diluent buffer
    Antibody diluent buffer
    ABC diluent buffer
    TMB color developing agent
    TMB stop solution
    Adhesive cover
  • Assay Time

    0.5 h

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

    Expiry Date

    12 months
  • Target See all AMBP ELISA Kits

    AMBP (alpha 1 Microglobulin/bikunin precursor (AMBP))

    Alternative Name

    AMBP

    Background

    Synonyms: Protein AMBP, Alpha-1-microglobulin, Protein HC, Alpha-1 microglycoprotein, Complex-forming glycoprotein heterogeneous in charge, Inter-alpha-trypsin inhibitor light chain, ITI-LC, Bikunin, EDC1, HI-30, Uronic-acid-rich protein, Trypstatin, AMBP, HCP, ITIL

    Tissue Specificity: Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha- trypsin inhibitor is present in plasma and urine.

    Background: Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

    Cellular Localisation: Secreted.

    UniProt

    P02760
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