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AMBP ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human AMBP.
Catalog No. ABIN5692240

Quick Overview for AMBP ELISA Kit (ABIN5692240)

Target

See all AMBP ELISA Kits
AMBP (alpha 1 Microglobulin/bikunin precursor (AMBP))

Binding Specificity

AA 206-352

Reactivity

  • 7
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6 pg/mL - 1000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Minimum Detection Limit

    15.6 pg/mL

    Purpose

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Bikunin. 96wells/kit, with removable strips.

    Brand

    PicoKine™

    Analytical Method

    Quantitative

    Specificity

    Expression system for standard: NSO, Immunogen sequence: A206-N352

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate precoated with antibody
    lyophilized recombinant standard
    biotinylated antibody (dilution 1:100)
    Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
    Sample diluent buffer
    Antibody diluent buffer
    ABC diluent buffer
    TMB color developing agent
    TMB stop solution
    Adhesive cover
  • Assay Time

    0.5 h

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

    Expiry Date

    12 months
  • Target See all AMBP ELISA Kits

    AMBP (alpha 1 Microglobulin/bikunin precursor (AMBP))

    Alternative Name

    AMBP

    Background

    Synonyms: Protein AMBP, Alpha-1-microglobulin, Protein HC, Alpha-1 microglycoprotein, Complex-forming glycoprotein heterogeneous in charge, Inter-alpha-trypsin inhibitor light chain, ITI-LC, Bikunin, EDC1, HI-30, Uronic-acid-rich protein, Trypstatin, AMBP, HCP, ITIL

    Tissue Specificity: Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha- trypsin inhibitor is present in plasma and urine.

    Background: Protein AMBP is a protein that in humans is encoded by the AMBP gene. This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

    Cellular Localisation: Secreted.

    UniProt

    P02760
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