beta 2 Defensin ELISA Kit
Quick Overview for beta 2 Defensin ELISA Kit (ABIN5706901)
Target
See all beta 2 Defensin (BD-2) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.156 ng/mL
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Analytical Method
- Quantitative
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Sensitivity
- 0.061 ng/mL
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual -
Sample Volume
- 100 µL
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Assay Time
- 1 - 4.5 h
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Plate
- Pre-coated
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Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Expiry Date
- 12 months
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- beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))
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Alternative Name
- Defensin Beta 2 (DEFb2)
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Background
- Alternative name: B-DF2, DEFB2, BD2, DEF-B2, HBD2, DEFB102, HBD-2, SAP1, DEFB4A, DEFB4, Skin-Antimicrobial Peptide 1
Target See all beta 2 Defensin (BD-2) ELISA Kits
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