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beta 2 Defensin ELISA Kit

Rat beta 2 Defensin ELISA Kit Colorimetric assay for quantification of Rat beta 2 Defensin.
Catalog No. ABIN5706901

Quick Overview for beta 2 Defensin ELISA Kit (ABIN5706901)

Target

See all beta 2 Defensin (BD-2) ELISA Kits
beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Reactivity

  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.156 ng/mL - 10 ng/mL

Application

ELISA

Sample Type

Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    0.156 ng/mL

    Analytical Method

    Quantitative

    Sensitivity

    0.061 ng/mL
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    Pre-coated, ready to use 96-well strip plate,
    Standard (freeze dried),
    Standard Diluent,
    Detection Reagent A,
    Detection Reagent B,
    Assay Diluent A,
    Assay Diluent B,
    TMB substrate,
    Stop Solution,
    Wash Buffer(30 x concenthumane),
    Plate sealer for 96 wells,
    Instruction manual

    Sample Volume

    100 µL

    Assay Time

    1 - 4.5 h

    Plate

    Pre-coated

    Protocol

    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Expiry Date

    12 months
  • Target See all beta 2 Defensin (BD-2) ELISA Kits

    beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

    Alternative Name

    Defensin Beta 2 (DEFb2)

    Background

    Alternative name: B-DF2, DEFB2, BD2, DEF-B2, HBD2, DEFB102, HBD-2, SAP1, DEFB4A, DEFB4, Skin-Antimicrobial Peptide 1
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