Myosin X ELISA Kit

Catalog No. ABIN5706904

Human Myosin X ELISA Kit Colorimetric assay for quantification of Human Myosin X.

Quick Overview for Myosin X ELISA Kit (ABIN5706904)

Target: Myosin X (MYO10)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details Myosin X ELISA Kit

Minimum Detection Limit: 0.312 ng/mL

Purpose: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MYO10 in human serum, plasma, tissue homogenates

Analytical Method: Quantitative

Sensitivity: 0.114 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 50 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5. Aspirate and wash 5 times
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for Myosin X

Target: Myosin X (MYO10)

Background: Alternative name: Unconventional Myosin-X, Unconventional Myosin-10