DOCK6 ELISA Kit

Catalog No. ABIN6211959

This Human DOCK6 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human DOCK6.

Quick Overview for DOCK6 ELISA Kit (ABIN6211959)

Target: DOCK6 (Dedicator of Cytokinesis 6 (DOCK6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details DOCK6 ELISA Kit

Minimum Detection Limit: 0.312 ng/mL

Purpose: Human Dedicator Of Cytokinesis Protein 6 (DOCK6) ELISA Kit

Analytical Method: Quantitative

Sensitivity: < 0.1 ng/mL

Components: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Material not included:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Application Details

Application Notes: Optimal dilutions/concentrations should be determined by the end user.

Comment:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plate: Pre-coated

Reagent Preparation:

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.

• 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.

• 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.

Sample Collection:

• Tissue homogenates: The preparation of tissue homogenates will vary depending upon tissue type - this is just an example. Rinse tissues with ice-cold PBS to remove the excess of blood. Weigh before homogenization. Finely mince tissues and homogenize with a tissue homogenizer on ice in PBS and sonicate the cell suspension. Centrifuge the homogenates at 5000 x g for 5 min and collect the supernatant. Assay immediately or aliquot and store at -20 °C.

• Cell lysates: Collect cells into a centrifuge tube and wash with PBS. Centrifuge the cell lysate at 1000 x g for 10 min and discard the supernatant. Add 300-500 μL PBS or NaCl per 1 x 10⁶ cells on ice and sonicate the cell suspension. Centrifuge at 10000 x g for 10 min at 4 °C, collect the supernatant and assay immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Expiry Date: 6 months

Target Details for DOCK6

Target: DOCK6 (Dedicator of Cytokinesis 6 (DOCK6))

Alternative Name: Dedicator Of Cytokinesis Protein 6

Background:

Ensembl: ENSG00000130158

UniProt Entry Name: DOCK6_HUMAN

Gene ID: 57572

HGNC: 19189

OMIM: 614194

UniProt: Q96HP0