CCL20 ELISA Kit

Catalog No. ABIN625049

This Human CCL20 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CCL20. This ELISA Kit has been cited in 6+ publications.

Quick Overview for CCL20 ELISA Kit (ABIN625049)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.5-600 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details CCL20 ELISA Kit

Minimum Detection Limit: 1.5 pg/mL

Purpose: Human MIP-3 alpha (CCL20) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensitivity: 1.5 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for CCL20 ELISA Kit (ABIN625049)

Wang, Su, Yang, Qiao, Fang, Yu, Yang, Wang, Yin, Chen, Hong: "The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery." in: International journal of cancer, Vol. 138, Issue 11, pp. 2688-99, (2016) (PubMed).

Crean, Cummins, Bahar, Mohan, McMorrow, Murphy: "Adenosine Modulates NR4A Orphan Nuclear Receptors To Attenuate Hyperinflammatory Responses in Monocytic Cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 4, pp. 1436-48, (2015) (PubMed).

Arranz-Valsero, Schulze, Contreras-Ruiz, García-Posadas, López-García, Paulsen, Diebold: "Involvement of corneal epithelial cells in the Th17 response in an in vitro bacterial inflammation model." in: Molecular vision, Vol. 19, pp. 85-99, (2013) (PubMed).

Iwata, Tanaka, Inoue, Toiyama, Hiro, Fujikawa, Okugawa, Uchida, Mohri, Kusunoki: "Macrophage inflammatory protein-3 alpha (MIP-3a) is a novel serum prognostic marker in patients with colorectal cancer." in: Journal of surgical oncology, Vol. 107, Issue 2, pp. 160-6, (2013) (PubMed).

Lee, Chiou, Tzeng, Chu: "Macrophage inflammatory protein-3alpha influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells." in: Toxicology, Vol. 249, Issue 2-3, pp. 116-22, (2008) (PubMed).

Weber: "[Metal ceramic boundary surfaces in the Ni-Cr-ceramic system from a recent viewpoint]." in: Deutsche zahnärztliche Zeitschrift, Vol. 37, Issue 11, pp. 896-9, (1983) (PubMed).

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: MIP-3 alpha

Background: Gene Names: CCL20 LARC MIP3A SCYA20
Protein names: C-C motif chemokine 20 (Beta-chemokine exodus-1) (CC chemokine LARC) (Liver and activation-regulated chemokine) (Macrophage inflammatory protein 3 alpha) (MIP-3-alpha) (Small-inducible cytokine A20) [Cleaved into: CCL20(1-67), CCL20(1-64), CCL20(2-70)]

Gene ID: 6364

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection