CCL20 ELISA Kit

Catalog No. ABIN625050

Human CCL20 ELISA Kit Colorimetric assay for quantification of Human CCL20.

Quick Overview for CCL20 ELISA Kit (ABIN625050)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.5-600 pg/mL

Application: ELISA

Sample Type: Tissue Lysate, Cell Lysate

Product Details CCL20 ELISA Kit

Minimum Detection Limit: 1.5 pg/mL

Purpose: Human MIP-3 alpha (CCL20) ELISA Kit for cell and tissue lysate samples.

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes human MIP-3 alpha.

Sensitivity: 1.5 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis
• Cell lysate buffer

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: MIP-3 alpha

Background: Gene Names: CCL20 LARC MIP3A SCYA20
Protein names: C-C motif chemokine 20 (Beta-chemokine exodus-1) (CC chemokine LARC) (Liver and activation-regulated chemokine) (Macrophage inflammatory protein 3 alpha) (MIP-3-alpha) (Small-inducible cytokine A20) [Cleaved into: CCL20(1-67), CCL20(1-64), CCL20(2-70)]

Gene ID: 6364

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection