MMP13 ELISA Kit

Catalog No. ABIN625055

Human MMP13 ELISA Kit Colorimetric assay for quantification of Human MMP13 and has been mentioned in 8+ publications.

Quick Overview for MMP13 ELISA Kit (ABIN625055)

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-6000 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details MMP13 ELISA Kit

Minimum Detection Limit: 6 pg/mL

Purpose: Human MMP-13 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested Human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.

Sensitivity: 6 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Sample Preparation:

Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for MMP13 ELISA Kit (ABIN625055)

Chijimatsu, Kunugiza, Taniyama, Nakamura, Tomita, Yoshikawa: "Expression and pathological effects of periostin in human osteoarthritis cartilage." in: BMC musculoskeletal disorders, Vol. 16, pp. 215, (2015) (PubMed).

Soydinç, Sak, Evliyaoğlu, Evsen, Turgut, Ozler, Tay, Gül: "Maternal plasma prolidase, matrix metalloproteinases 1 and 13, and oxidative stress levels in pregnancies complicated by preterm premature rupture of the membranes and chorioamnionitis." in: Journal of the Turkish German Gynecological Association, Vol. 13, Issue 3, pp. 172-7, (2014) (PubMed).

Zhang, Zhu, Chen, Zhang, Zhang, Jia, Wang, Liu, Xiao: "Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes." in: International immunopharmacology, Vol. 21, Issue 2, pp. 301-8, (2014) (PubMed).

Balasubramanian, Pye, Willcox: "Effects of eye rubbing on the levels of protease, protease activity and cytokines in tears: relevance in keratoconus." in: Clinical & experimental optometry : journal of the Australian Optometrical Association, Vol. 96, Issue 2, pp. 214-8, (2013) (PubMed).

Yuan, Zhao, Yuan, Che, Duan, Liu, Dong: "Association of endothelin-1 expression and cartilaginous endplate degeneration in humans." in: PLoS ONE, Vol. 8, Issue 4, pp. e60062, (2013) (PubMed).

Rhim, Ahn, Kim, Kim, Lee, Kim, Kim, Park: "Stimulation of matrix metalloproteinases by tumor necrosis factor-? in human pulp cell cultures." in: Journal of endodontics, Vol. 39, Issue 6, pp. 795-800, (2013) (PubMed).

Toegel, Wu, Otero, Goldring, Leelapornpisid, Chiari, Kolb, Unger, Windhager, Viernstein: "Caesalpinia sappan extract inhibits IL1?-mediated overexpression of matrix metalloproteinases in human chondrocytes." in: Genes & nutrition, Vol. 7, Issue 2, pp. 307-18, (2012) (PubMed).

Jacob, Eisemon, Sheibani-Rad, Patel, Jacob, Choueka: "Matrix metalloproteinase levels as a marker for rotator cuff tears." in: Orthopedics, Vol. 35, Issue 4, pp. e474-8, (2012) (PubMed).

Target Details for MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: MMP-13

Background: Gene Names: MMP13
Protein names: Collagenase 3 (EC 3.4.24.-) (Matrix metalloproteinase-13) (MMP-13)

Gene ID: 4322

UniProt: P45452