TIMP2 ELISA Kit

Catalog No. ABIN625100

Human TIMP2 ELISA Kit Colorimetric assay for quantification of Human TIMP2 and has been mentioned in 20+ publications.

Quick Overview for TIMP2 ELISA Kit (ABIN625100)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 25-7000 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details TIMP2 ELISA Kit

Minimum Detection Limit: 25 pg/mL

Purpose: Human TIMP-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, PDGF, SCF, TNF-alpha, TNF-beta, TPO, VEGF.

Sensitivity: 2 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples300 - 3,000 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for TIMP2 ELISA Kit (ABIN625100)

Prato, Khadjavi, Magnetto, Gulino, Rolfo, Todros, Cavalli, Guiot: "Effects of oxygen tension and dextran-shelled/2H,3H-decafluoropentane-cored oxygen-loaded nanodroplets on secretion of gelatinases and their inhibitors in term human placenta." in: Bioscience, biotechnology, and biochemistry, Vol. 80, Issue 3, pp. 466-72, (2016) (PubMed).

Gulino, Magnetto, Khadjavi, Panariti, Rivolta, Soster, Argenziano, Cavalli, Giribaldi, Guiot, Prato: "Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes." in: Mediators of inflammation, Vol. 2015, pp. 964838, (2015) (PubMed).

Garratt, Sutanto, Ling, Looi, Iosifidis, Martinovich, Shaw, Kicic-Starcevich, Knight, Ranganathan, Stick, Kicic: "Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis." in: The European respiratory journal, Vol. 46, Issue 2, pp. 384-94, (2015) (PubMed).

Khadjavi, Magnetto, Panariti, Argenziano, Gulino, Rivolta, Cavalli, Giribaldi, Guiot, Prato: "Chitosan-shelled oxygen-loaded nanodroplets abrogate hypoxia dysregulation of human keratinocyte gelatinases and inhibitors: New insights for chronic wound healing." in: Toxicology and applied pharmacology, Vol. 286, Issue 3, pp. 198-206, (2015) (PubMed).

Ciaffi, Cavassini, Genne, Delhumeau, Spycher Elbes, Hill, Wandeler, Fehr, Stoeckle, Schmid, Hirschel, Montecucco, Calmy: "Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study." in: European journal of clinical investigation, Vol. 45, Issue 7, pp. 720-30, (2015) (PubMed).

Basilico, Magnetto, DAlessandro, Panariti, Rivolta, Genova, Khadjavi, Gulino, Argenziano, Soster, Cavalli, Giribaldi, Guiot, Prato: "Dextran-shelled oxygen-loaded nanodroplets reestablish a normoxia-like pro-angiogenic phenotype and behavior in hypoxic human dermal microvascular endothelium." in: Toxicology and applied pharmacology, Vol. 288, Issue 3, pp. 330-8, (2015) (PubMed).

Koob, Lim, Zabek, Massee: "Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 5, pp. 1133-40, (2015) (PubMed).

Kiliç, Uçar, Özdemir, Colak, Bal, Ertu?rul: "Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions." in: Anadolu kardiyoloji dergisi : AKD = the Anatolian journal of cardiology, Vol. 14, Issue 6, pp. 531-41, (2014) (PubMed).

Richards, Timulak, Doherty, Sharry, Colla, Joyce, Hayes: "Internet-delivered treatment: its potential as a low-intensity community intervention for adults with symptoms of depression: protocol for a randomized controlled trial." in: BMC psychiatry, Vol. 14, pp. 147, (2014) (PubMed).

Hampel, Klonisch, Sel, Schulze, Garreis, Seitmann, Zouboulis, Paulsen: "Insulin-like factor 3 promotes wound healing at the ocular surface." in: Endocrinology, Vol. 154, Issue 6, pp. 2034-45, (2013) (PubMed).

Chon, Kim, Ahn: "Transient elastography in real practice: a time for action?" in: Journal of gastroenterology and hepatology, Vol. 27, Issue 7, pp. 1134-5, (2012) (PubMed).

Kaminska-Winciorek, Deja, Polanska, Jarosz-Chobot: "The role of selected metalloproteinases in cheiroarthropathy in children with type 1 diabetes - a pilotage study." in: International journal of clinical practice, Vol. 66, Issue 4, pp. 374-7, (2012) (PubMed).

Müller, Kainov, El Bakkouri, Saelens, De Brabander, Kittel, Samm, Muller: "The proton translocation domain of cellular vacuolar ATPase provides a target for the treatment of influenza A virus infections." in: British journal of pharmacology, Vol. 164, Issue 2, pp. 344-57, (2012) (PubMed).

Mamlouk, Balagurumoorthy, Wang, Adelstein, Kassis: "Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes." in: International journal of radiation biology, Vol. 88, Issue 12, pp. 1019-27, (2012) (PubMed).

Moir, Ng, Poniris, Santa, Burgess, Oliver, Krymskaya, Black: "Doxycycline inhibits matrix metalloproteinase-2 secretion from TSC2-null mouse embryonic fibroblasts and lymphangioleiomyomatosis cells." in: British journal of pharmacology, Vol. 164, Issue 1, pp. 83-92, (2012) (PubMed).

Steed, Trumpower, Duffy, Smith, Marshall, Rupp, Robson: "Amnion-derived cellular cytokine solution: a physiological combination of cytokines for wound healing." in: Eplasty, Vol. 8, pp. e18, (2008) (PubMed).

Malard, Berenguer, Prat, Ruat, Steinmetz, Quemeneur: "Global gene expression profiling in human lung cells exposed to cobalt." in: BMC genomics, Vol. 8, pp. 147, (2007) (PubMed).

Burns, Griffith, Waldhausen: "A linear oscillating electromotor for possible application in an intrathoracic artificial heart." in: Transactions - American Society for Artificial Internal Organs, Vol. 10, pp. 151-3 (PubMed).

Hickman, Sargent: "The application of the use of radioactive phosphorus (32P) in larvae: a new approach for identification of adult insect sources." in: Investigative radiology, Vol. 1, Issue 2, pp. 104-5 (PubMed).

Jay: "Production of lysozyme by staphylococci and its correlation with three other extracellular substances." in: Journal of bacteriology, Vol. 91, Issue 5, pp. 1804-10 (PubMed).

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP-2

Background: Gene Names: TIMP2
Protein names: Metalloproteinase inhibitor 2 (CSC-21K) (Tissue inhibitor of metalloproteinases 2) (TIMP-2)

Gene ID: 7077

UniProt: P16035

Pathways: cAMP Metabolic Process