C5A ELISA Kit

Catalog No. ABIN629495

Product Details C5A ELISA Kit

Target: C5A (C5a) (Complement Component 5a (C5a))

Reactivity: Human

Method Type: Sandwich ELISA

Application: ELISA

Characteristics: ELISA kit for detection of Complement C5 a in the research laboratory
Alternative Names: C5a ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

The C5a ELISA KIT is an enzyme immunoassay for the in vitro determination of human anaphylatoxin C5a and is based on the sandwich principle. Due to cross-reactivity of the monoclonal antibodies with complement factor C5, C5 in the sample is removed by precipitation prior to analysis. The resulting clear supernatant contains the C5a to be determined . During the first incubation the C5a in the sample binds to Mouse anti C5a monoclonal antibodies which are attached to the surface of the microtitration plate. Unbound constituents are then removed by washing and, in a second reaction, peroxidase conjugated monoclonal antibodies (Mab 557) are added and bound to a different epitope on C5a. The excess enzyme conjugated antibodies are removed by washing, the bound enzyme activity is then determined. The enzymatic reaction between hydrogen peroxide and chromogen is terminated by the addition of dilute sulphuric acid. The intensity of the colour intensity, which is proportional to the concentration of C5a, is determined photometrically. The concentration range of appox. 0.1 to 10 µg/L (the exact values are indicated on the labels) is covered by the standards contained in the kit. For higher concentrations the sample must be diluted with dilution reagent.

Restrictions: For Research Use only

Handling

Precaution of Use: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV I/II, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Storage: 4 °C

Target Details for C5A

Target: C5A (C5a) (Complement Component 5a (C5a))

Alternative Name: Complement C5a (C5a Products)

Background: Upon activation, a cascade of proteolytic enzymes releases the anaphylatoxins C3a, C4a and C5a from their respective precursors. These fragments exert various biological functions such as histamine release, smooth muscle contraction, increase in capillary permeability or immunomodulation. In addition, C5a and its degraded form C5a-desArg are highlypotent chemotactic agents for polymorphonuclear leukocytes, which then will release tissue degradative enzymes and oxygen radicals.

Pathways: Complement System, Carbohydrate Homeostasis