F11R ELISA Kit

Catalog No. ABIN6719688

Supplier Product No.: koa0813

This Human F11R ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human F11R. This ELISA Kit has been cited in1 publication.

Quick Overview for F11R ELISA Kit (ABIN6719688)

Target: F11R (F11 Receptor (F11R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.9 pg/mL - 3000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details F11R ELISA Kit

Minimum Detection Limit: 46.9 pg/mL

Supplier Product No.: koa0813

Supplier: Rockland

Purpose: Human JAM-A Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human JAM-A. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 46.9pg/mL-3000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL human JAM-A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants or serum to each empty well. We recommend that each human JAM-A standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: S28-V238

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for F11R ELISA Kit (ABIN6719688)

Bluhmki, Bitzer, Gindele, Schruf, Kiechle, Webster, Schymeinsky, Ries, Gantner, Bischoff, Garnett, Heilker: "Development of a miniaturized 96-Transwell air-liquid interface human small airway epithelial model." in: Scientific reports, Vol. 10, Issue 1, pp. 13022, (2020) (PubMed).

Target Details for F11R

Target: F11R (F11 Receptor (F11R))

Alternative Name: JAM-A

Background:

Synonyms: CD 321, CD321, CD321 antigen, ESTM33, F11 receptor, F11R, JAM 1, JAM A, JAM, JAM-1, JAM-A, JAM1, JAM1_HUMAN, JAMA, JCAM, Jcam1, Junction adhesion molecule 1, Junction adhesion molecule mouse homolog of, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, Ly106, PAM 1, PAM-1, PAM1, Platelet adhesion molecule 1, Platelet adhesion molecule, Platelet F11 receptor, PRO301, UNQ264, UNQ264/PRO301

Background: Junctional adhesion molecule A(JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Gene ID: 50848

NCBI Accession: NP_058642

UniProt: Q9Y624

Pathways: Cell-Cell Junction Organization