Resistin ELISA Kit
Quick Overview for Resistin ELISA Kit (ABIN6719732)
Target
See all Resistin (RETN) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 78 pg/mL
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Supplier Product No.
- koa0355
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Supplier
- Rockland
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Purpose
- Human Resistin Sandwich ELISA Kit for Quantitative Detection
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Brand
- AccuSignal™
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Analytical Method
- Quantitative
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Specificity
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Production: Natural and recombinant human Resistin. There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 3 pg/mL
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Components
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Application Notes
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Resistin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. We recommend that each human Resistin standard solution and each sample is measured in duplicate.
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Comment
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Standard: Expression system for standard: E.coli, Immunogen sequence: K19-P108
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Sample Volume
- 100 μL
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- Resistin (RETN)
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Alternative Name
- Resistin
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Background
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Synonyms: Adipose tissue specific secretory factor, Adipose tissue-specific secretory factor, ADSF, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1, Cysteine rich secreted protein A12 alpha like 2, Cysteine rich secreted protein FIZZ3, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3, Found in inflammatory zone 3, Resistin, Resistin delta2, RETN_HUMAN, RETN1, XCP 1, XCP1
Background: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin(for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
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Gene ID
- 56729
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NCBI Accession
- NP_001180303
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UniProt
- Q9HD89
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Pathways
- Feeding Behaviour, Smooth Muscle Cell Migration
Target See all Resistin (RETN) ELISA Kits
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