Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Resistin ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Resistin.
Rockland
Catalog No. ABIN6719732
Supplier Product No.: koa0355

Quick Overview for Resistin ELISA Kit (ABIN6719732)

Target

See all Resistin (RETN) ELISA Kits
Resistin (RETN)

Reactivity

  • 7
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

78 pg/mL - 5000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Minimum Detection Limit

    78 pg/mL

    Supplier Product No.

    koa0355

    Supplier

    Rockland

    Purpose

    Human Resistin Sandwich ELISA Kit for Quantitative Detection

    Brand

    AccuSignal™

    Analytical Method

    Quantitative

    Specificity

    Production: Natural and recombinant human Resistin. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 3 pg/mL

    Components

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Application Notes

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Resistin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. We recommend that each human Resistin standard solution and each sample is measured in duplicate.

    Comment

    Standard: Expression system for standard: E.coli, Immunogen sequence: K19-P108

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Expiry Date

    12 months
  • Target See all Resistin (RETN) ELISA Kits

    Resistin (RETN)

    Alternative Name

    Resistin

    Background

    Synonyms: Adipose tissue specific secretory factor, Adipose tissue-specific secretory factor, ADSF, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1, Cysteine rich secreted protein A12 alpha like 2, Cysteine rich secreted protein FIZZ3, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3, Found in inflammatory zone 3, Resistin, Resistin delta2, RETN_HUMAN, RETN1, XCP 1, XCP1

    Background: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance.1 Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin(for resistance to insulin), that may be the hormone potentially links obesity to diabetes.2 Resistin is overexpressed in human adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.3 The standard product used in this kit is human Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

    Gene ID

    56729

    NCBI Accession

    NP_001180303

    UniProt

    Q9HD89

    Pathways

    Feeding Behaviour, Smooth Muscle Cell Migration
You are here:
Chat with us!