XCL1 ELISA Kit

Catalog No. ABIN6719776

Supplier Product No.: koa0475

Mouse XCL1 ELISA Kit Colorimetric assay for quantification of Mouse XCL1.

Quick Overview for XCL1 ELISA Kit (ABIN6719776)

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details XCL1 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Supplier Product No.: koa0475

Supplier: Rockland

Purpose: Mouse XCL1 - Lymphotactin Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse XCL1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 62.5pg/mL-4000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse XCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse XCL1 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: V22-G114

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for XCL1

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Alternative Name: XCL1/Lymphotactin

Background:

Synonyms: ATAC, C motif chemokine 1, Chemokine (C motif) ligand 1, Cytokine SCM-1, Cytokine SCM1, LPTN, LTN, Lymphotactin, Lymphotaxin, SCM 1, SCM 1 alpha, SCM-1-alpha, SCM1, SCM1 alpha, SCYC1, Small inducible cytokine C1, Small inducible cytokine subfamily C member 1, Small-inducible cytokine C1, XC chemokine ligand 1, Xcl1, XCL1_HUMAN

Background: Chemokine(C motif) ligand(XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

Gene ID: 16963

NCBI Accession: NP_032536

UniProt: P47993

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation