EpCAM ELISA Kit

Catalog No. ABIN6719777

Supplier Product No.: koa0446

This Colorimetric ELISA kit is designed for the quantitative measurement of Human EpCAM.

Quick Overview for EpCAM ELISA Kit (ABIN6719777)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.7 pg/mL - 6000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details EpCAM ELISA Kit

Minimum Detection Limit: 93.7 pg/mL

Supplier Product No.: koa0446

Supplier: Rockland

Purpose: Human EPCAM Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human EPCAM. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human EPCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human EPCAM standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: Q24-K265

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EPCAM

Background:

Synonyms: 17 1A, 323/A3, AUA1, CD326, CD326 antigen, CO 17A, CO17 1A, CO17A, DIAR5, EGP2, EGP, EGP2, EGP314, EGP40, Ep CAM, Ep-CAM, EPCAM, ESA, GA733 1, GA733 2, GA733-2, hEGP 2, hEGP314, HNPCC8, KS1/4, KSA, M1S 1, M1S2 ,M4S1, MIC18 MK 1 ,TACD1, TACSTD1, TROP1

Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. Also it is involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since it is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.

Gene ID: 4072

NCBI Accession: NP_002345

UniProt: P16422