Soluble Tumor Necrosis Factor Receptor Type 2 (sTNF-R2) ELISA Kit

Catalog No. ABIN6719786

Supplier Product No.: koa0322

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Soluble Tumor Necrosis Factor Receptor Type 2.

Quick Overview for Soluble Tumor Necrosis Factor Receptor Type 2 (sTNF-R2) ELISA Kit (ABIN6719786)

Target: Soluble Tumor Necrosis Factor Receptor Type 2 (sTNF-R2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine

Product Details

Minimum Detection Limit: 7.8 pg/mL

Supplier Product No.: koa0322

Supplier: Rockland

Purpose: Human sTNFsR II Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human sTNFsRII. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human sTNFsRII standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA, citrate) or urine to each empty well. We recommend that each human sTNFsRII standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: L23-D257

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Soluble Tumor Necrosis Factor Receptor Type 2 (sTNF-R2)

Alternative Name: sTNFsR II

Background:

Synonyms: CD120b, Etanercept, p75, p80 TNF alpha receptor, TBP2, TBPII, TNF R2, TNFBR, TNFR-II, TNFR2, TNFRII, Tumor necrosis factor binding protein 2, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor superfamily member 1b, membrane form, Tumor necrosis factor receptor type II

Background: Tumor necrosis factor receptor p75(TNF-R p75, or TNF-R2) is a 75- kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II(TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.

Gene ID: 7133

NCBI Accession: NP_001057

UniProt: P20333