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TIMP3 ELISA Kit

This Human TIMP3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human TIMP3.
Rockland
Catalog No. ABIN6719796
Supplier Product No.: koa0315

Quick Overview for TIMP3 ELISA Kit (ABIN6719796)

Target

See all TIMP3 ELISA Kits
TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivity

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Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

156 pg/mL - 10000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
  • Minimum Detection Limit

    156 pg/mL

    Supplier Product No.

    koa0315

    Supplier

    Rockland

    Purpose

    Human TIMP-3 Sandwich ELISA Kit for Quantitative Detection

    Brand

    AccuSignal™

    Analytical Method

    Quantitative

    Specificity

    Production: Natural and recombinant human TIMP-3. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 2 pg/mL

    Components

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Application Notes

    ELISA_Dilution: 156pg/mL-10000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TIMP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin , EDTA) or saliva to each empty well. It is recommended that each human TIMP-3 standard solution and each sample be measured in duplicate.

    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: C24-P211

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Expiry Date

    12 months
  • Target See all TIMP3 ELISA Kits

    TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

    Alternative Name

    TIMP-3

    Background

    Synonyms: HSMRK222, K222, K222TA2, Metalloproteinase inhibitor 3, MIG 5 protein, Protein MIG-5, SFD, TIMP metallopeptidase inhibitor 3, TIMP-3, TIMP3, TIMP3_HUMAN, Tissue inhibitor of metalloproteinases 3

    Background: The tissue inhibitors of metalloproteinases(TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy(SFD).

    Gene ID

    7078

    NCBI Accession

    NP_000353

    UniProt

    P35625
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