IL23 ELISA Kit

Catalog No. ABIN6719809

Supplier Product No.: koa0524

Mouse IL23 ELISA Kit Colorimetric assay for quantification of Mouse IL23.

Quick Overview for IL23 ELISA Kit (ABIN6719809)

Target: IL23 (Interleukin 23 (IL23))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Product Details IL23 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0524

Supplier: Rockland

Purpose: Mouse IL-23 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse IL-23. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IL-23 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatant, serum or plasma (heparin) to each empty well. It is recommended that each mouse IL-23 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: sf21, Immunogen sequence: (M23-S419)+(L20-A196)

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL23

Target: IL23 (Interleukin 23 (IL23))

Alternative Name: IL-23

Background:

Synonyms: IL 23, IL 23A, IL-23 subunit alpha, IL-23-A, IL-23p19, Il23a, IL23A_HUMAN, IL23P19, Interleukin 23 alpha subunit p19, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, JKA3 induced upon T cell activation, P19, SGRF

Background: Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19(the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12(stSG47812).

Gene ID: 83430

NCBI Accession: NP_112542

UniProt: Q9EQ14