CD32-B/C ELISA Kit

Catalog No. ABIN6719821

Supplier Product No.: koa0785

Human CD32-B/C ELISA Kit, Colorimetric assay for quantification of Human CD32-B/C.

Quick Overview for CD32-B/C ELISA Kit (ABIN6719821)

Target: CD32-B/C

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.9 pg/mL - 3000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details

Minimum Detection Limit: 46.9 pg/mL

Supplier Product No.: koa0785

Supplier: Rockland

Purpose: Human CD32 - FCGR2b - c Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CD32. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL human CD32 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CD32 standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: A46-P217

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: CD32-B/C

Alternative Name: CD32/FCGR2b/c

Background:

Synonyms: CD32 antigen, CD32A, CD32B, CD32C, CDw32, Fc fragment of IgG low affinity IIa receptor, Fc fragment of IgG low affinity IIb receptor, Fc fragment of IgG low affinity IIc receptor, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, FC-gamma-RIIB, FCG2, FCG2A_HUMAN, FcgammaRIIC, FcGR, FCGR2, FCGR2A, FCGR2C, Fcgr3, Fcgr3a, Fcr-2, Fcr-3, FcRII a, FCRII, FcRII b, FcRIIC, IGFR 2, IGFR2, IgG Fc receptor II a, IgG Fc receptor II a, Low affinity immunoglobulin gamma Fc region receptor II, Lymphocyte antigen 17

Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Gene ID: 9103

NCBI Accession: NP_963857

UniProt: P31995