NUCB2 ELISA Kit

Catalog No. ABIN6719848

Supplier Product No.: koa0652

Human NUCB2 ELISA Kit Colorimetric assay for quantification of Human NUCB2.

Quick Overview for NUCB2 ELISA Kit (ABIN6719848)

Target: NUCB2 (NES1) (Nesfatin-1 (NES1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details NUCB2 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0652

Supplier: Rockland

Purpose: Human Nesfatin-1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse CCL8. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Nesfatin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human Nesfatin-1 standard solution and each sample be measured in duplicate. Seal the plate with the cover and incubate at 37 °C for 90 min.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: V25-L106

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for NUCB2

Target: NUCB2 (NES1) (Nesfatin-1 (NES1))

Alternative Name: Nesfatin-1

Background:

Synonyms: DNA binding protein NEFA, DNA-binding protein NEFA, Gastric cancer antigen Zg4, NEFA, Nefastin 1, Nefastin-1, Nefastin1, Nucb2, NUCB2_HUMAN, Nucleobindin-2, Nucleobinding 2, Prepronefastin, Prepronesfatin

Background: Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2(NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.

Gene ID: 4925

NCBI Accession: NP_005004

UniProt: P80303