CCL8 ELISA Kit

Catalog No. ABIN6719955

Supplier Product No.: koa0651

This Mouse CCL8 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse CCL8.

Quick Overview for CCL8 ELISA Kit (ABIN6719955)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CCL8 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Supplier Product No.: koa0651

Supplier: Rockland

Purpose: Mouse CCL8 - MCP2 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse CCL8. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CCL8 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CCL8 standard solution and each sample is measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: E20-P97

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Alternative Name: CCL8/MCP2

Background:

Synonyms: C C motif chemokine 8, CCL 8, Ccl8, CCL8_HUMAN, Chemokine (C C motif) ligand 8, Chemokine ligand 8, HC14, MCP 2, MCP-2, MCP-2(6-76), Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, SCYA10, SCYA8, Small inducible cytokine A8, Small inducible cytokine subfamily A (Cys Cys) member 8, Small-inducible cytokine A8

Background: Chemokine(C-C motif) ligand 8, also known as monocyte chemoattractant protein 2(MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2(MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

Gene ID: 20307

NCBI Accession: NP_067418

UniProt: Q9Z121