CD21 ELISA Kit

Catalog No. ABIN6720009

Supplier Product No.: koa0788

This Human CD21 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human CD21.

Quick Overview for CD21 ELISA Kit (ABIN6720009)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CD21 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Supplier Product No.: koa0788

Supplier: Rockland

Purpose: Human CD21 - CR2 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CD21. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 20 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human CD21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD21 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: CHO, Immunogen sequence: Ile21-71

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21/CR2

Background:

Synonyms: C3DR, CD 21, CD21, CD21 antigen, Complement C3d receptor, Complement component (3d/Epstein Barr virus) receptor 2, Complement component receptor 2, Complement receptor type 2, Cr 2, CR, Cr2, CR2_HUMAN, CVID7, EBV receptor, EBV-R, Epstein Barr virus receptor, Epstein-Barr virus receptor, EVBR, SLEB9

Background: Complement receptor type 2(CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b(inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).

Gene ID: 1380

NCBI Accession: NP_001006659

UniProt: P20023

Pathways: Complement System