Prokineticin 1 ELISA Kit

Catalog No. ABIN6720042

Supplier Product No.: koa0351

This Human Prokineticin 1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Prokineticin 1.

Quick Overview for Prokineticin 1 ELISA Kit (ABIN6720042)

Target: Prokineticin 1 (Prok1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details Prokineticin 1 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Supplier Product No.: koa0351

Supplier: Rockland

Purpose: Human EG-VEGF Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human EG-VEGF. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 7 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human EG-VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human EG-VEGF standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: A20-F105

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Prokineticin 1

Target: Prokineticin 1 (Prok1)

Alternative Name: EG-VEGF

Background:

Synonyms: EG-VEGF, Endocrine-gland-derived vascular endothelial growth factor, Mambakine, PROK1, PROK1_HUMAN, Prokineticin-1

Background: EG-VEGF, Endocrine gland derived vascular endothelial growth factor, is a human protein encoded by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF(A20-F105), with molecular weight of 10.5 KDa.

Gene ID: 84432

NCBI Accession: NP_115790

UniProt: P58294