HFE2 ELISA Kit

Catalog No. ABIN6720086

Product Details HFE2 ELISA Kit

Target: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: DL M,HEMOCHROMATOSIS, HEMOCHROMATOSIS DUE TO DEFECT IN HEMOJUVELIN, HEMOCHROMATOSIS DUE TO DEFECT IN HEPCIDIN ANTIMICROBIAL PEPTIDE, HEMOCHROMATOSIS JUVENILE, Hemochromatosis type 2, Hemochromatosis type 2 protein, Hemochromatosis type 2 protein homolog, HEMOCHROMATOSIS TYPE 2A, HEMOCHROMATOSIS TYPE 2B, HFE2A, HJV, JH, Juvenile, Repulsive guidance molecule c, RGM C, RGM domain family member C, RGMC

Background: Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of a three gene family (in vertebrates) called the repulsive guidance molecules. In mouse, the Hemojuvelin protein is encoded by the HFE2 gene, and it is mapped to Chromosome 3. It was concluded that downregulation of hepatic Hemojuvelin during inflammation may induce a temporary elimination of iron sensing. Hemojuvelin is a coreceptor for bone morphogenetic proteins (BMPs), and inhibition of endogenous BMP signaling reduces hepcidin expression and increases serum iron in mice. Furthermore, Hemojuvelin may play inhibitory roles in prostate cancer by suppressing cell growth, adhesion, migration and invasion.

Gene Name: HFE2

Production: Natural and recombinant mouse Hemojuvelin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q33-D393

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Hemojuvelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Hemojuvelin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for HFE2

Target: HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))

Alternative Name: Hemojuvelin (HFE2 Products)

Synonyms: HFE2A ELISA Kit, HJV ELISA Kit, JH ELISA Kit, RGMC ELISA Kit, HFE2 ELISA Kit, DKFZp468F2322 ELISA Kit, 2310035L15Rik ELISA Kit, 5230400G09Rik ELISA Kit, AI414844 ELISA Kit, AI789733 ELISA Kit, DL-M ELISA Kit, Rgmc ELISA Kit, hemojuvelin ELISA Kit, RGMr ELISA Kit, hjv ELISA Kit, id:ibd3464 ELISA Kit, wu:fb38f10 ELISA Kit, zgc:136698 ELISA Kit, hemochromatosis type 2 (juvenile) ELISA Kit, hemochromatosis type 2 ELISA Kit, HFE2 ELISA Kit, Hfe2 ELISA Kit, hfe2 ELISA Kit

Gene ID: 69585

NCBI Accession: NP_081402

UniProt: Q7TQ32

Pathways: Transition Metal Ion Homeostasis