Endoglin ELISA Kit

Catalog No. ABIN6720098

Supplier Product No.: koa0371

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Endoglin.

Quick Overview for Endoglin ELISA Kit (ABIN6720098)

Target: Endoglin (ENG)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details Endoglin ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0371

Supplier: Rockland

Purpose: Human CD105 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CD105. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 15 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD105 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD105 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: CHO, Immunogen sequence: E26-G586

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105

Background:

Synonyms: AI528660, AI662476, CD 105, CD105 antigen, Cell surface MJ7/18 antigen, END, Endoglin, ENG, FLJ41744, HHT1, ORW, ORW1, Osler Rendu Weber syndrome 1, OTTMUSP00000012838, OTTMUSP00000012839, RP11 228B15.2, RP23 17P12.4, S endoglin, Transmembrane glycoprotein

Background: CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta(TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant huma...

Gene ID: 2022

NCBI Accession: NP_000109

UniProt: P17813