IDS ELISA Kit

Catalog No. ABIN6720131

Supplier Product No.: koa0877

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IDS.

Quick Overview for IDS ELISA Kit (ABIN6720131)

Target: IDS (Iduronate 2-Sulfatase (IDS))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details IDS ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0877

Supplier: Rockland

Purpose: Mouse IDS Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse IDS. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 15 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse IDS standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IDS standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: T36-P552

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IDS

Target: IDS (Iduronate 2-Sulfatase (IDS))

Alternative Name: IDS

Background:

Synonyms: Ids, Iduronate 2 sulfatase, Iduronate sulfatase, Idursulfase, MPS2, SIDS

Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Gene ID: 15931

NCBI Accession: NP_034628

UniProt: Q08890

Pathways: Glycosaminoglycan Metabolic Process