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TIMP2 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat TIMP2.
Rockland
Catalog No. ABIN6720140
Supplier Product No.: koa0776

Quick Overview for TIMP2 ELISA Kit (ABIN6720140)

Target

See all TIMP2 ELISA Kits
TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity

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Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

156 pg/mL - 10000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Minimum Detection Limit

    156 pg/mL

    Supplier Product No.

    koa0776

    Supplier

    Rockland

    Purpose

    Rat TIMP-2 Sandwich ELISA Kit for Quantitative Detection

    Brand

    AccuSignal™

    Analytical Method

    Quantitative

    Specificity

    Production: Natural and recombinant rat TIMP-2. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 10 pg/mL

    Components

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Application Notes

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL rat TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each rat TIMP-2 standard solution and each sample is measured in duplicate.

    Comment

    Standard: Expression system for standard: NSO, Immunogen sequence: C27-P220

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Expiry Date

    12 months
  • Target See all TIMP2 ELISA Kits

    TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

    Alternative Name

    TIMP-2

    Background

    Synonyms: CSC 21K, CSC21K, D11Bwg1104e, DDC8, Metalloproteinase inhibitor 2, Metalloproteinase inhibitor 2 precursor, MGC105282, OTTMUSP00000004170, OTTMUSP00000004171, RP23-394O9.2, TIMP 2, TIMP metallopeptidase inhibitor 2, Tissue inhibitor of metalloproteinase 2, Tissue inhibitor of metalloproteinases 2

    Background: TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

    Gene ID

    29543

    NCBI Accession

    NP_068824

    UniProt

    P30121

    Pathways

    cAMP Metabolic Process
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